{"auditDate":"2026-07-26","scope":"Public parasite single-cell or single-nucleus transcriptomics with accessible cell-level expression and metadata","result":"No qualifying parasite single-cell dataset was identified for the seven evidence-gap taxa below.","caveat":"This is a dated, source-bounded search result rather than proof that no unpublished or newly released dataset exists.","taxa":[{"slug":"tbg","species":"Trypanosoma brucei gambiense","atlasStatus":"evidence-gap","closestEvidence":"GSE18065","evidenceType":"bulk digital gene-expression libraries","scope":"Six parasite libraries: three procyclic and three bloodstream-form biological replicates.","exclusion":"Bulk libraries do not provide cell-resolved expression, cell metadata or single-cell coordinates.","primaryUrl":"https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE18065","repositoryUrl":"https://www.ncbi.nlm.nih.gov/sra/?term=SRP001797","repositoryLabel":"SRA SRP001797"},{"slug":"tbr","species":"Trypanosoma brucei rhodesiense","atlasStatus":"evidence-gap","closestEvidence":"PMID 29474390","evidenceType":"bulk transcriptomes","scope":"Parasites sampled from patient blood and cerebrospinal fluid, with rat and culture comparisons.","exclusion":"The publication reports population-level transcriptomes rather than cell-resolved parasite profiles.","primaryUrl":"https://pubmed.ncbi.nlm.nih.gov/29474390/","repositoryUrl":"https://doi.org/10.1371/journal.pntd.0006280","repositoryLabel":"PLOS Neglected Tropical Diseases"},{"slug":"tco","species":"Trypanosoma congolense","atlasStatus":"evidence-gap","closestEvidence":"GSE165290","evidenceType":"bulk RNA-seq","scope":"Sixteen RNA-seq samples spanning two conditions, two species and four replicates per combination.","exclusion":"Samples are bulk populations. A separate mouthpart-transcriptomics paper explicitly describes parasite single-cell RNA-seq as future work.","primaryUrl":"https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE165290","repositoryUrl":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9063227/","repositoryLabel":"Mouthpart transcriptomics paper"},{"slug":"tvi","species":"Trypanosoma vivax","atlasStatus":"evidence-gap","closestEvidence":"BioProject PRJNA144513","evidenceType":"bulk lifecycle RNA-seq and proteomics","scope":"Global gene-expression profiling across the T. vivax lifecycle.","exclusion":"Lifecycle profiles are population averages and do not expose per-cell expression or single-cell embeddings.","primaryUrl":"https://pubmed.ncbi.nlm.nih.gov/26266535/","repositoryUrl":"https://www.ncbi.nlm.nih.gov/bioproject/144513","repositoryLabel":"NCBI BioProject 144513"},{"slug":"tev","species":"Trypanosoma evansi","atlasStatus":"evidence-gap","closestEvidence":"GSE202334 and GSE184265","evidenceType":"expression microarray and bulk RNA-seq","scope":"GSE202334 exposes 64 array samples; GSE184265 includes triplicate bulk RNA-seq samples for T. evansi, T. equiperdum and T. brucei.","exclusion":"Neither record contains cell-resolved parasite expression suitable for a single-cell atlas.","primaryUrl":"https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE202334","repositoryUrl":"https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE184265","repositoryLabel":"GEO GSE184265"},{"slug":"teq","species":"Trypanosoma equiperdum","atlasStatus":"evidence-gap","closestEvidence":"GSE184265","evidenceType":"bulk RNA-seq","scope":"Triplicate population RNA-seq samples for T. equiperdum alongside T. evansi and T. brucei.","exclusion":"The experimental unit is a bulk parasite population, not an individual cell.","primaryUrl":"https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE184265","repositoryUrl":"https://www.ncbi.nlm.nih.gov/sra/?term=SRP337410","repositoryLabel":"SRA SRP337410"},{"slug":"tra","species":"Trypanosoma rangeli","atlasStatus":"evidence-gap","closestEvidence":"NCBI Taxonomy 5698","evidenceType":"genome and gene records; no qualifying expression atlas","scope":"The species has public taxonomy, genome and gene resources, but the portal search did not identify an accessible parasite single-cell transcriptomics record.","exclusion":"Genome annotation is a reference resource, not cell-level expression evidence.","primaryUrl":"https://www.ncbi.nlm.nih.gov/taxonomy/5698","repositoryUrl":"https://www.ncbi.nlm.nih.gov/datasets/genome/?taxon=5698","repositoryLabel":"NCBI genome records"}]}