SPECIES EVIDENCE DIRECTORY · 2026-07-26

没有合格单细胞数据，也要展示真实证据

这里逐物种记录系统检索中发现的最接近公开数据，并说明为什么它不能作为寄生虫单细胞图谱。证据缺口不会被占位点图或推测性细胞标签填补。

AUDIT DATE2026-07-26

SEARCH SCOPEPublic parasite single-cell or single-nucleus transcriptomics with accessible cell-level expression and metadata

RESULT7 evidence gaps

This is a dated, source-bounded search result rather than proof that no unpublished or newly released dataset exists.

EVIDENCE GAP

Trypanosoma brucei gambiense

GSE18065

Closest public evidence: bulk digital gene-expression libraries
Verified scope: Six parasite libraries: three procyclic and three bloodstream-form biological replicates.
Why it is not an atlas: Bulk libraries do not provide cell-resolved expression, cell metadata or single-cell coordinates.

EVIDENCE GAP

PMID 29474390

Closest public evidence: bulk transcriptomes
Verified scope: Parasites sampled from patient blood and cerebrospinal fluid, with rat and culture comparisons.
Why it is not an atlas: The publication reports population-level transcriptomes rather than cell-resolved parasite profiles.

EVIDENCE GAP

GSE165290

Closest public evidence: bulk RNA-seq
Verified scope: Sixteen RNA-seq samples spanning two conditions, two species and four replicates per combination.
Why it is not an atlas: Samples are bulk populations. A separate mouthpart-transcriptomics paper explicitly describes parasite single-cell RNA-seq as future work.

EVIDENCE GAP

BioProject PRJNA144513

Closest public evidence: bulk lifecycle RNA-seq and proteomics
Verified scope: Global gene-expression profiling across the T. vivax lifecycle.
Why it is not an atlas: Lifecycle profiles are population averages and do not expose per-cell expression or single-cell embeddings.

EVIDENCE GAP

GSE202334 and GSE184265

Closest public evidence: expression microarray and bulk RNA-seq
Verified scope: GSE202334 exposes 64 array samples; GSE184265 includes triplicate bulk RNA-seq samples for T. evansi, T. equiperdum and T. brucei.
Why it is not an atlas: Neither record contains cell-resolved parasite expression suitable for a single-cell atlas.

EVIDENCE GAP

GSE184265

Closest public evidence: bulk RNA-seq
Verified scope: Triplicate population RNA-seq samples for T. equiperdum alongside T. evansi and T. brucei.
Why it is not an atlas: The experimental unit is a bulk parasite population, not an individual cell.

EVIDENCE GAP

NCBI Taxonomy 5698

Closest public evidence: genome and gene records; no qualifying expression atlas
Verified scope: The species has public taxonomy, genome and gene resources, but the portal search did not identify an accessible parasite single-cell transcriptomics record.
Why it is not an atlas: Genome annotation is a reference resource, not cell-level expression evidence.